The science of
cryopreservation.

Cryopreservation means cooling biological material to temperatures where biochemical activity effectively halts. Stored below roughly −130°C, well-preserved tissue can remain intact for decades or longer instead of continuing to degrade.

Clinical cryopreservation already works routinely for embryos, sperm, eggs, stem cells, and some tissues. The frontier is scale: preserving complexity, vascular networks, and coordinated organ function without structural compromise.

Modern cryobiology spans fertility clinics, organ transplant logistics, cell therapy manufacturing, and biobanking at scale. What was once limited to small samples and simple tissues is now pushing toward whole organs and, in the laboratory, whole organisms.

Recent advances in organ vitrification, vascular perfusion, and controlled rewarming are closing the gap between what works in a dish and what works at the scale of a kidney, a heart, or an entire circulatory system. Integrated organ and tissue preservation could transform transplantation, regenerative medicine, and public health on a scale comparable to curing cancer.

One of the clearest demonstrations of that shift is CryoDAO-funded work on vitrified whole sheep ovaries: perfusion through a proprietary device, ice-free storage at liquid nitrogen temperatures, nanowarming, and replantation. That work shows a complex vascularized organ from a large mammal can recover full function after cryogenic storage, on a path toward the first FDA-approved vitrified whole organ.

Preserving a whole organism means preserving circulation, organ systems, and tissue architecture together. The work is systems engineering at that scale: perfusing cryoprotectants through the vasculature, controlling cooling and rewarming uniformly across tissues, and preventing ice formation or thermal stress fractures throughout the body.

Laboratory progress is measured in steps. Renal vitrification with transplantation and long-term survival showed that a vital mammalian organ can survive the process end to end. Multi-organ models in large mammals test whether several tissues can be preserved and rewarmed together.

The CRYORAT project, funded by CryoDAO, is one of the most direct tests of reversibility at the whole-body scale: high sub-zero preservation and revival of a small mammal. That is the direction Noah and our research partners are pushing: rigorous protocols, reproducible outcomes, and scale that matches real biology rather than isolated samples.

The same core science that preserves organs in the lab applies wherever life must survive extremes: trauma, distance, time, and uncertainty. That is what Noah is building: Cryopets in the field today, and research that pushes whole-organism cryopreservation toward human biostasis. Done well, preservation retains the biological structure and information future medicine will need to restore.

We believe whole-organism cryopreservation is the most consequential technology of our time. It is the bridge between two ambitions that have defined human striving for as long as we have looked up at the night sky: defeating death, and reaching the stars.

That is why Noah exists. We are here to bring reversible human cryopreservation to the world. The science is hard, but the payoff is eternal.

Decades of peer-reviewed work underpins reversible cryopreservation for whole organisms, from early brain viability studies to organ vitrification, memory retention after cooling, and connectome preservation. Explore some of the most impactful papers in the field below.